Cell unroofing encompasses various methods used to isolate and expose the cell membrane of cells. Unlike the more common membrane extraction protocols performed with multiple steps of centrifugation (which aims to separate the membrane fraction from a cell lysate), cell unroofing aims to tear and preserve patches of the plasma membrane to perform in situ experiments using microscopy and biomedical spectroscopy.
Cell unroofing encompasses various methods used to isolate and expose the cell membrane of cells. Unlike the more common membrane extraction protocols performed with multiple steps of centrifugation (which aims to separate the membrane fraction from a cell lysate), cell unroofing aims to tear and preserve patches of the plasma membrane to perform in situ experiments using microscopy and biomedical spectroscopy.
Source: Wikipedia "Cell unroofing" · CC BY-SA 4.0
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