Gene trapping is a high-throughput approach that is used to introduce insertional mutations across an organism's genome. == Method == Trapping is performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene and/or selectable genetic marker, flanked by an upstream 3' splice site (splice acceptor; SA) and a downstream transcriptional termination sequence (polyadenylation sequence; polyA).
Gene trapping is a high-throughput approach that is used to introduce insertional mutations across an organism's genome. == Method == Trapping is performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene and/or selectable genetic marker, flanked by an upstream 3' splice site (splice acceptor; SA) and a downstream transcriptional termination sequence (polyadenylation sequence; polyA).
Source: Wikipedia "Gene trapping" · CC BY-SA 4.0
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