Hanes–Woolf plot
In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of a / v {\displaystyle a/v} against a {\displaystyle a} is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration a {\displaystyle a} to the reaction velocity v {\displaystyle v} is plotted against a {\displaystyle a} . It is based on the rearrangement of the Michaelis–Menten equation shown below: a v = a V + K m V {\displaystyle {a \over v}={a \over V}+{K_{\mathrm {m} } \over V}} where K m {\displaystyle K_{\mathrm {m} }} is the Michaelis constant and V {\displaystyle V} is the limiting rate.