Isothermal titration calorimetry

In chemical thermodynamics, isothermal titration calorimetry (ITC) is a physical technique used to determine the thermodynamic parameters of interactions in solution. ITC is the only technique capable of comprehensively characterizing thermodynamic and kinetic profiles of a molecular interaction by simultaneously determining binding constants ( K a {\displaystyle K_{a}} ), reaction stoichiometry ( n {\displaystyle n} ), enthalpy ( Δ H {\displaystyle \Delta H} ), Gibbs free energy ( Δ G {\displaystyle \Delta G} ) and entropy ( Δ S {\displaystyle \Delta S} ) within a single experiment.

Source: Wikipedia — Isothermal titration calorimetry (CC BY-SA 4.0)