Selector technique

The selector technique is a method to amplify and multiplex genomic DNA. == Process == Genomic DNA is digested with restriction enzymes, circularized by hybridisation to selectors and subsequently attached to a vector sequence by ligation. The procedure results in circular DNA molecules with an included general primer pair motif that can be used for amplification by PCR or RCA. == Selector construct == A selector consists of two oligonucleotides, one Vector oligonucleotide and one Selector probe.

Source: Wikipedia — Selector technique (CC BY-SA 4.0)

Selector technique

The selector technique is a method to amplify and multiplex genomic DNA. == Process == Genomic DNA is digested with restriction enzymes, circularized by hybridisation to selectors and subsequently attached to a vector sequence by ligation. The procedure results in circular DNA molecules with an included general primer pair motif that can be used for amplification by PCR or RCA. == Selector construct == A selector consists of two oligonucleotides, one Vector oligonucleotide and one Selector probe.

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Source: Wikipedia "Selector technique" · CC BY-SA 4.0

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